团队介绍----广州海洋实验室

团队介绍

一、团队简介

海洋是巨大的微生物资源宝库。海洋微生物涵盖了细菌、古菌、真菌、病毒以及微藻等多种类型。几十来，海洋微生物的生物多样性和潜在应用价值日益受到关注。本团队围绕海洋微生物，特别是具有重要生物学、生态学或开发应用价值的微生物类群，开展分子生物学研究，同时收集和挖掘微生物资源，从而促进对海洋微生物的生物学认知和生物技术开发。

二、研究方向

1.海洋分子微生物学：在分子、组学等水平解析重要海洋微生物（含病毒）的基本生物学特征、遗传代谢机制、生态功能、环境适应性和演化规律等。

2.海洋微生物资源：发掘具有生物技术应用潜力的海洋微生物及其产物。

三、研究团队：

PI：黄力

邮箱：huang_li@gmlab.ac.cn

个人简介：

黄力，研究员，美国微生物科学院院士（AAM Fellow）、国家杰出青年基金获得者、中国科学院“百人计划”入选者、基金委创新群体学术带头人、全国优秀科技工作者。曾任微生物资源前期开发国家重点实验室主任、微生物所常务副所长、所长、中国微生物学会副理事长、基金委生命科学部咨询委员会委员。现任基金委“水圈微生物驱动地球元素循环的机制”重大研究计划指导专家组副组长以及mLife共同主编、Extremophiles执行主编。长期从事海洋微生物资源、古菌遗传机制及其环境适应机制、古菌病毒等研究。

研究方向：海洋微生物、古菌遗传机制及古菌病毒

近年主持和参加的主要项目:

1.基金委重大项目（32393970）2024-2028，“古菌功能演化与真核生物起源”中课题二任务。

2.科技部重点研发计划项目“极端微生物底盘细胞的设计与构建”中课题“耐热、耐酸微生物底盘细胞的设计构建与表征”的任务（2020YFA0906800）2020-2025。

3.基金委面上基金 (31970170). 2020-2023，古菌纺锤形病毒SSV_PH感染循环关键步骤的分子机制。

4.中国科学院B类先导专项”印太交汇区海洋物质能量中心形成演化过程与机制”子课题 “深海微生物分布地理格局及形成机制”（XDB42030302）2020-2024。

5.基金委重点基金（31730001）2018-2022，古菌真核类型引发酶的亚基性质与功能演化的研究。

6.科技部重点研发计划项目“深海热液区生物资源研究与应用评价”课题“深海热液区病毒多样性及其与宿主互作机制研究”（2018YFC0310703）2018-2021。

7.中国大洋协会“十三五”资源环境课题“深海稀有微生物物种和海洋病毒及其基因资源的调查、获取与发掘”（DY135-B-02）2016-2020。

8.基金委面上基金（31470175）2015-2018，古菌蛋白质甲基转移酶aKMT的催化机理及生理功能。

9.基金委面上基金（31270123）2013-2016，一种新的古菌引物合成体系的生化性质及生理功能。

10.基金委重点基金（31130003）2012-2016，极端嗜热古菌小分子核酸结合蛋白的研究。

11.基金委创新研究群体基金（30921065）2010-2012，极端环境微生物生命特征及环境适应机理。

科研成果：

（1）代表性论文：

1.Wang S, She Q, Huang L*. 2022. Sulfolobus islandicus Employs Orc1-2-Mediated DNA Damage Response in Defense against Infection by SSV2. J Virol. 96(24):e0143822. doi: 10.1128/jvi.01438-22.

2.Han Z, Yuan W, Xiao H, Wang L, Zhang J, Peng Y, Cheng L*, Liu H*, Huang L*. 2022. Structural insights into a spindle-shaped archaeal virus with a sevenfold symmetrical tail. Proc Natl Acad Sci U S A. 119(31):e2119439119. doi: 10.1073/pnas.2119439119.

3.Ding N, Chen Y, Chu Y, Zhong C, Huang L*, Zhang Z. 2022. Lysine Methylation Modulates the Interaction of Archaeal Chromatin Protein Cren7 With DNA. Front Microbiol. 13:837737. doi: 10.3389/fmicb.2022.837737.

4.Li J, Zheng X, Li L, Zhang S, Ren M, Huang L, Dong X. 2022. The Archaeal Transcription Termination Factor aCPSF1 is a Robust Phylogenetic Marker for Archaeal Taxonomy. Microbiol Spectr. 9(3):e0153921. doi: 10.1128/spectrum.01539-21.

5.Zheng X, Dai X, Zhu Y, Yang J, Jiang H, Dong H, Huang L*. 2022. (Meta)Genomic Analysis Reveals Diverse Energy Conservation Strategies Employed by Globally Distributed Gemmatimonadota. mSystems. 7(4):e0022822. doi: 10.1128/msystems.00228-22.

6.Liu W, Zheng X, Dai X, Zhang Z, Zhang W, Xiao T, Huang L*. 2022. Isolation and Characterization of the First Temperate Virus Infecting Psychrobacillus from Marine Sediments. Viruses. 14(1):108. doi: 10.3390/v14010108.

7.Huang L*, Wang H, Ictv Report Consortium. 2021. ICTV Virus Taxonomy Profile: Ovaliviridae. J Gen Virol. 102(3):001546. doi: 10.1099/jgv.0.001546.

8.Dai X, Wang Y, Luo L, Pfiffner SM, Li G, Dong Z, Xu Z, Dong H, Huang L*. 2021. Detection of the deep biosphere in metamorphic rocks from the Chinese continental scientific drilling. Geobiology. 19(3):278-291. doi: 10.1111/gbi.12430.

9.Zhang J, Huang L*. 2021. Construction of a Fusellovirus with a Minimal Set of Genes. ACS Synth Biol. 10(10):2617-2627. doi: 10.1021/acssynbio.1c00232. Epub 2021 Sep 20.

10.Huang L, Zhang Z, McMacken R. 2021. Interaction of the Escherichia coli HU Protein with Various Topological Forms of DNA. Biomolecules. 11(11):1724. doi: 10.3390/biom11111724.

11.Zhang N, Guo L, Huang L*. 2020. The Sac10b homolog from Sulfolobus islandicus is an RNA chaperone. Nucleic Acids Res. 48(16):9273-9284. doi: 10.1093/nar/gkaa656.

12.Zhang Z, Zhan Z, Wang B, Chen Y, Chen X, Wan C, Fu Y*, Huang L*. 2020. Archaeal chromatin proteins Cren7 and Sul7d compact DNA by bending and bridging. mBio. 11(3):e00804-20.

13.Zheng X, Liu W, Dai X, Zhu Y, Wang J, Zhu Y, Zheng H, Huang Y, Dong Z, Du W, Zhao F, Huang L*. 2020. Extraordinary diversity of viruses in deep-sea sediments as revealed by metagenomics without prior virion separation. Environ Microbiol. doi: 10.1111/1462-2920.15154. Epub ahead of print. PMID: 32627268.

14.Ruan CJ, Wang J, Zheng XW, Song L, Zhu YX, Huang Y, Lu ZJ, Du WB, Huang L*, Dai X*. 2020. Halovulum marinum sp. nov., isolated from deep-sea water of the Indian Ocean, and emended description of the genus Halovulum. Int J Syst Evol Microbiol. doi: 10.1099/ijsem.0.004307. Epub ahead of print. PMID: 32589573.

15.Wang J, Ruan CJ, Song L, Li A, Zhu YX, Zheng XW, Wang L, Lu ZJ, Huang Y, Du W, Zhou Y, Huang L*, Dai X*. 2020. Gimesia benthica sp. nov., a planctomycete isolated from a deep-sea water sample of the Northwest Indian Ocean. Int J Syst Evol Microbiol. doi: 10.1099/ijsem.0.004301. Epub ahead of print. PMID: 32589565.

16.Zhang J, Zheng X, Wang H, Jiang H, Dong H, Huang L*. 2020. Novel Sulfolobus Fuselloviruses with Extensive Genomic Variations. J Virol. 94(4):e01624-19.

17.Yun J, Zheng X, Xu P, Zheng X, Xu J, Cao C, Fu Y, Xu B, Dai X, Wang Y, Liu H, Yi Q, Zhu Y, Wang J, Wang L, Dong Z, Huang L*, Huang Y*, Du W*. 2020. Interfacial nanoinjection-based nanoliter single-cell analysis. Small. 16(9):e1903739.

18.Huang L*, Xiao X*, Zhao G*. 2019. Microbes: the hidden giant behind the biogeochemical cycling of elements in the hydrosphere. Sci China Life Sci. 62(10):1271-1274.

19.Cao J, Wang T, Wang Q, Zheng X, Huang L*. 2019. Functional Insights Into Protein Acetylation in the Hyperthermophilic Archaeon Sulfolobus islandicus. Mol Cell Proteomics. 18(8):1572-1587.

20.Dong X, Li M, Xiang H, Xu J, Wang F, Shen Y, Zhang Z, Han J, Li J, Li M, Huang L*. 2019. Unraveling the secrets of the third form of life—A review and future perspective on archaeal research in China. SCIENTIA SINICA Vitae. 49(11):1520-1542. (Chinese)

21.Ruan CJ, Zheng XW, Wang J, Song L, Zhu YX, Du WB, Lu ZJ, Huang Y, Huang L*, Dai X*. 2018. Hyphobacterium indicum sp. nov., isolated from deep seawater, and emended description of the genus Hyphobacterium. Int J Syst Evol Microbiol. 68(12):3760-3765.

22.Zhang Z, Zhao M, Chen Y, Wang L, Liu Q, Dong Y, Gong Y*, Huang L*. 2019. Architectural roles of Cren7 in folding crenarchaeal chromatin filament. Mol Microbiol. 111(3):556-569.

23.Cao J, Wang Q, Liu T, Peng N, Huang L*. 2018. Insights into the post-translational modifications of archaeal Sis10b (Alba): lysine-16 is methylated, not acetylated, and this does not regulate transcription or growth. Mol Microbiol. doi: 10.1111/mmi.13973.

24.Wang H, Guo Z, Feng H, Chen Y, Chen X, Li Z, Hernández-Ascencio W, Dai X, Zhang Z, Zheng X, Mora-López M, Fu Y, Zhang C, Zhu P*, Huang L*. 2018. Novel Sulfolobus virus with an exceptional capsid architecture. J Virol. 92(5):e01727-17.

25.Zhang J, Pan B, Li Z, Sheng Zhao X*, Huang L*. 2017. Kinetic insights into the temperature dependence of DNA strand cleavage and religation by topoisomerase III from the hyperthermophile Sulfolobus solfataricus. Sci Rep. 7(1):5494.

26.Huang L*, Feng X, Du Q, Dong X, Liu S, Wen M, Dai X. 2017. Focusing on key scientific issues of microbiome research in hydrosphere: NSFC major research plan for microbes in hydrosphere. Bull Chin Acad Sci. 32(03):266-272. (Chinese)

27.Zhang Z, Zhao M, Wang L, Chen Y, Dong Y, Gong Y*, Huang L*. 2017. Roles of Leu28 side chain intercalation in the interaction between Cren7 and DNA. Biochem J. 474(10):1727-1739.

28.Dai X, Wang H, Zhang Z, Li K, Zhang X, Mora-López M, Jiang C, Liu C, Wang L, Zhu Y, Hernández-Ascencio W, Dong Z, Huang L*. 2016. Genome Sequencing of Sulfolobus sp. A20 from Costa Rica and Comparative Analyses of the Putative Pathways of Carbon, Nitrogen, and Sulfur Metabolism in Various Sulfolobus Strains. Front Microbiol. 7:1902.

29.Sun F, Huang L*. 2016. A euryarchaeal histone modulates strand displacement synthesis by replicative DNA polymerases. Sci China Life Sci. 59(7):709-16.

30.Chu Y, Zhu Y, Chen Y, Li W, Zhang Z, Liu D, Wang T, Ma J, Deng H, Liu ZJ, Ouyang S*, Huang L*. 2016. aKMT catalyzes extensive protein lysine methylation in the hyperthermophilic archaeon Sulfolobus islandicus but is dispensable for the growth of the organism. Mol Cell Proteomics. 15(9):2908-23.

31.Liu B, Ouyang S, Makarova KS, Xia Q, Zhu Y, Li Z, Guo L, Koonin EV, Liu ZJ*, Huang L*. 2015. A primase subunit essential for efficient primer synthesis by an archaeal eukaryotic-type primase. Nat Commun. 6:7300.

32.Zhan Z, Zhou J, Huang L. 2015*. Site-specific recombination by SSV2 integrase: substrate requirement and domain functions. J Virol. 89:10934-44.

33.Lang S, Huang L*. 2015. The Sulfolobus solfataricus GINS complex stimulates DNA binding and processive DNA unwinding by minichromosome maintenance helicase. J Bacteriol. 197:3409-20.

34.Zheng X, Liu H, Song L, Zhang L, Wang H, Dai X, Huang L*. 2015. Phaeocystidibacter marisrubri sp. nov., a member of the family Cryomorphaceae isolated from Red Sea sediment. Int J Syst Evol Microbiol. 65:2199-203.

35.Wang H, Peng N, Shah SA, Huang L*, She Q*. 2015. Archaeal extrachromosomal genetic elements. Microbiol Mol Biol Rev. 79:117-52.

36.Xiang X, Huang X, Wang H, Huang L*. 2015. pTC plasmids from Sulfolobus species in the geothermal area of Tengchong, China: genomic conservation and naturally-occurring variations as a result of transposition by mobile genetic elements. Life (Basel). 5:506-20.

37.Zhang Z, Gong Y, Chen Y, Li H, Huang L*. 2015. Insights into the interaction between Cren7 and DNA: the role of loop β3-β4. Extremophiles. 19:395-406.

38.Guo L, Ding J, Guo R, Hou Y, Wang DC*, Huang L*. 2014. Biochemical and structural insights into RNA binding by ssh10b, a member of the highly conserved sac10b protein family in archaea. J Biol Chem. 289:1478-90.

39.Song L, Wang H, Wang S, Zhang H, Cong H, Huang L*, Tien P*. 2014. Self-assembled bionanoparticles based on the Sulfolobus tengchongensis spindle-shaped virus 1 (STSV1) coat protein as a prospective bioscaffold for nanotechnological applications. Extremophiles. 18:745-54.

40.Xing X, Zhang L, Guo L, She Q, Huang L*. 2014. Sulfolobus replication factor C stimulates the activity of DNA polymerase B1. J Bacteriol. 196(13):2367-75.

41.Erdmann S, Chen B, Huang X, Deng L, Liu C, Shah SA, Le Moine Bauer S, Sobrino CL, Wang H, Wei Y, She Q, Garrett RA, Huang L*, Lin L*. 2014. A novel single-tailed fusiform Sulfolobus virus STSV2 infecting model Sulfolobus species. Extremophiles. 18(1):51-60.

42.Sun F, Huang L*. 2013. Sulfolobus chromatin proteins modulate strand displacement by DNA polymerase B1. Nucleic Acids Res. 41(17):8182-95.

43.Ren Y, She Q, Huang L*. 2013. Transcriptomic analysis of the SSV2 infection of Sulfolobus solfataricus with and without the integrative plasmid pSSVi. Virology. 441(2) 126-34.

44.Hu J, Guo L, Wu K, Liu B, Lang S, Huang L*. 2012. Template-dependent polymerization across discontinuous templates by the heterodimeric primase from the hyperthermophilic archaeon Sulfolobus solfataricus. Nucleic Acids Res. 40(8):3470-83.

45.Chu Y, Zhang Z, Wang Q, Luo Y, Huang L*. 2012. Identification and characterization of a highly conserved crenarchaeal protein lysine methyltransferase with broad substrate specificity. J Bacteriol. 194(24):6917-26.

46.Zhan Z, Ouyang S, Liang W, Zhang Z, Liu ZJ*, Huang L*. 2012. Structural and functional characterization of the C-terminal catalytic domain of SSV1 integrase. Acta Crystallogr D Biol Crystallogr. 68(Pt 6):659-70.

47.Dai X, Zhu Y, Luo Y, Song L, Liu D, Liu L, Chen F, Wang M, Li J, Zeng X, Dong Z, Hu S, Li L, Xu J*, Huang L*, Dong X*. 2012. Metagenomic insights into the fibrolytic microbiome in yak rumen. PLoS One. 7(7):e40430.

48.Zhang Z, Guo L, Huang L*. 2012. Archaeal chromatin proteins. Sci China Life Sci. 55(5):377-85. Review.

49.Guo L, Brügger K, Liu C, Shah SA, Zheng H, Zhu Y, Wang S, Lillestøl RK, Chen L, Frank J, Prangishvili D, Paulin L, She Q, Huang L*, Garrett RA*. 2011. Genome analyses of Icelandic strains of Sulfolobus islandicus, model organisms for genetic and virus-host interaction studies. J Bacteriol. 193(7):1672-80.

50.Li X, Guo L, Deng L, Feng D, Ren Y, Chu Y, She Q*, Huang L*. 2011. Deletion of the topoisomerase III gene in the hyperthermophilic archaeon Sulfolobus islandicus results in slow growth and defects in cell cycle control. J Genet Genomics. 38(6):253-9.

51.Zhang Z, Gong Y, Guo L, Jiang T, Huang L*. 2010. Structural insights into the interaction of the crenarchaeal chromatin protein Cren7 with DNA. Mol. Microbiol. 76(3):749-59.

52.Guo X, Huang L*. 2010. An SF3 DNA helicase encoded by plasmid pSSVi from the hyperthermophilic Archaeon Sulfolobus solfataricus unwinds DNA as a higher-order oligomer and interacts with host primase. J. Bacteriol, 192(7):1853-64.

53.Zhang L, Lou H, Guo L, Zhan Z, Duan Z, Guo X, Huang L*. 2010. Accurate DNA synthesis by Sulfolobus solfataricus DNA polymerase B1 at high temperature. Extremophiles, 14: 107-17.

54.Liu Y, Guo L, Guo R, Wong RL, Hernandez H, Hu J, Chu Y, Amster IJ, Whitman WB*, Huang L*. 2009. The Sac10b homolog in Methanococcus maripaludis binds DNA at specific sites. J Bacteriol. 191(7):2315-29.

55.Guo L, Feng F, Zhang Z, Yao H, Luo Y, Wang J, Huang L*. 2008. Biochemical and structural characterization of Cren7, a novel chromatin protein conserved among Crenarchaea. Nucleic Acids Res. 36: 1129-37.

56.Wang S, Liu WF, He YZ, Zhang A, Huang L*, Dong ZY*, Yan YB*. 2008. Multistate folding of a hyperthermostable Fe-superoxide dismutase (TcSOD) in guanidinium hydrochloride: The importance of the quaternary structure. Biochim Biophys Acta. 1784: 445-54.

57.Wang Y, Duan Z, Zhu H, Guo X, Wang Z, Zhou J, She Q, Huang L*. 2007. A novel Sulfolobus non-conjugative extrachromosomal genetic element capable of integration into the host genome and spreading in the presence of a fusellovirus. Virology 363: 124-33.

58.He Y, Fan K, Jia C, Wang Z, Pan W, Huang L*, Yang K*, Dong Z*. 2007. Characterization of a hyperthermostable Fe-superoxide dismutase from hot spring. Appl. Microbiol. Biotechnol. 75:367-76.

59.Chen L, Huang L*. 2006. Oligonucleotide cleavage and rejoining by topoisomerase III from the hyperthermophilic archaeon Sulfolobus solfataricus: temperature dependence and strand annealing-promoted DNA religation. Mol. Microbiol. 60:783-94.

60.Wu K, Lai X, Guo X, Hu J, Xiang X, Huang L*. 2006. Interplay between primase and replication factor C in the hyperthermophilic archaeon Sulfolobus solfataricus. Mol. Microbiol. 63(3):826–837.

61.Wang Y, Xia Y, Dong X, Dong H, Yang K, Dong Z, Huang L. 2006. Quantitative analysis of two uncultured bacterial species from the terrestrial deep subsurface by real-time PCR. Acta Petrol Sin. 22(9):2103-2106. (Chinese)

62.Xiang X, Chen N, Huang X, Luo Y, She Q*, Huang L*. 2005. Spindle-shaped virus STSV1: virus-host interactions and genomic features. J. Virol. 79:8677-86.

63.Lai X, Huang L*. 2005. Biochemical properties of DNA ligase from the hyperthermophilic archaeon Sulfolobus shibatae. Prog Biochem Biophys. 32(2):122-128. (Chinese)

64.Lou H, Duan Z, Sun T, Huang L*. 2004. Cleavage of double-stranded DNA by the intrinsic 3’-5’ exonuclease activity of DNA polymerase B1 from the hyperthermophilic archaeon Sulfolobus solfataricus at high temperature. FEMS Microbiol Lett. 231(1):111-7.

65.Lou H, Duan Z, Huo X, Huang L*. 2004. Modulation of hyperthermophilic DNA polymerase activity by archaeal chromatin proteins. J Biol Chem. 279:127-32.

66.Guo R, Xue H, Huang L*. 2003. Ssh10b, a conserved thermophilic archaeal protein, binds RNA in vivo. Mol. Microbiol. 50:1605-15.

67.Xiang X, Dong X, Huang L*. 2003. Sulfolobus tengchongensis sp. nov., a novel thermoacidophilic archaeon isolated from a hot spring in Tengchong, China. Extremophiles 7:493-8.

68.Dai P, Wang Y, Ye R, Chen L, Huang L*. 2003. DNA topoisomerase III from the hyperthermophilic archaeon Sulfolobus solfataricus with specific DNA cleavage activity. J. Bacteriol. 185:5500-7.

69.Dai P, Huang L*. 2003. Purification of a reverse gyrase from the hyperthermophilic archaeon Sulfolobus shibatae using a simple procedure. Wei Sheng Wu Xue Bao (Acta Microbiologica Sinica) 43:241-4. (Chinese)

70.Lai X, Shao H, Hao F, Huang L*. 2002. Biochemical characterization of an ATP-dependent DNA ligase from the hyperthermophilic crenarchaeon Sulfolobus shibatae. Extremophiles 6: 469-77.

71.Chen X, Guo R, Huang L*. 2002. Evolutionary conservation and DNA binding properties of the Ssh7 proteins from Sulfolobus shibatae. Sci. China Ser. C. 45:583-92.

72.Liu D, Huang L*. 2002. Induction of the Sulfolobus shibatae virus SSV1 DNA replication by mitomycin C. Chinese Sci. Bull. 47: 923-27.

73.Li F, Hao F, Huang L*. 2001. Purification and characterization of a DNA helicase from the hyperthermophilic archaeon Sulfolobus shibatae. Wei Sheng Wu Xue Bao (Acta Microbiologica Sinica) 41:340-7. (Chinese)

74.Wang X, Tang Y, Wang J, Huang Y, Chen R, Huang L*. 2001. Extraction of DNA from environmental samples and construction of mixed genomic DNA libraries. Wei Sheng Wu Xue Bao (Acta Microbiologica Sinica) 41:133-40. (Chinese)

75.Xue H, Guo R, Wen Y, Liu D, Huang L*. 2000. An abundant DNA binding protein from the hyperthermophilic archaeon Sulfolobus shibatae affects DNA supercoiling in a temperature-dependent fashion. J. Bacteriol. 182:3929-33.

76.Chen X, Tang Y, Huang L*. 2000. Overproduction and partial characterization of the Ssh7a and Ssh7b proteins from Sulfolobus shibatae. Wei Sheng Wu Xue Bao (Acta Microbiologica Sinica) 40:359-64. (Chinese)

77.Lou H，Huang L*, Mai V. 1999. Effect of DNA binding protein Ssh12 from hyperthermophilic archaeon Sulfolobus shibatae on DNA supercoiling. Sci China Ser. C, 42:401-8.

78.Mai V, Chen X, Hong R, Huang L*. 1998. Small abundant DNA binding proteins from the thermoacidophilic archaeon Sulfolobus shibatae constrain negative DNA supercoils. J. Bacteriol. 180:2560-3.

79.Huang L, McGavin M, Forsberg C*, Lam J, Cheng K. 1990. Antigenic nature of the chloride-stimulated cellobiosidase and other cellulases of Fibrobacter succinogenes subsp. succinogenes S85 and related fresh isolates. Appl. Environ. Microbiol. 56:1229-34.

80.Huang L, Forsberg C*. 1990. Cellulose digestion and cellulase regulation and distribution in Fibrobacter succinogenes subsp. succinogenes S85. Appl. Environ. Microbiol. 56:1221-8.

81.Huang L, Forsberg C*, Thomas D. 1988. Purification and characterization of a chloride-stimulated cellobiosidase from Bacteroides succinogenes S85. J. Bacteriol. 170:2923-32.

82.Huang L, Forsberg C*. 1988. Purification and comparison of the periplasmic and extracellular forms of cellobiosidase from Bacteroides succinogenes. Appl. Environ. Microbiol. 54:1488-93.

83.Huang L, Forsberg C*. 1987. Isolation of a cellodextrinase from Bacteroides succinogenes. Appl. Environ. Microbiol. 53:1034-41.

84.Huang L, Forsberg C*, Gibbins L. 1986. Influence of external pH gradient and membrane potential in Clostridium acetobutylicum intracellular pH and cellular distribution of fermentation products. Appl. Environ. Microbiol. 51:1230-4.

85.Huang L, Gibbins L, Forsberg C*. 1985. Transmembrane pH gradient and membrane potential in Clostridium acetobutylicum under acetogenic and solventogenic conditions. Appl. Environ. Microbiol. 50:1043-7.

（2）代表性国际会议报告

1.Huang L. TBA. The 14th International Congress on Extremophiles, September 22-26, 2024, Loutraki, Greece.

2.Huang, L., Han, Z., Wang, S., Yuan, W., Xiao, H., Wang, L., Zhang, J., Peng, Y., She, Q., Cheng, L., and Liu, H. Novel insights into the structure and host-interaction of fuselloviruses. Viruses of Microbes 2023, July 3-7, 2023, Tbilisi, Geogia.

3.Huang L. Functional insights into post-translational modifications in Sulfolobus islandicus. The 15th Thermophiles Conference, September 2-6, 2019, Fukuoka, Japan.

4.Huang L. A major Chinese research initiative on microbes in the hydrosphere. 6th International Workshop on Deep-Sea Microbiology, October 3-5, 2018, Busan, South Korea.

5.Huang L. Insights into the functions of the N-acetyltransferase Pat and the N-terminal acetyltransferase Ard1 from Sulfolobus islandicus. The 12th International Congress on Extremophiles, September 16-20, 2018, Ischia (Naples), Italy.

6.Huang L. An archaeal methyltransferase catalyzes extensive protein methylation but is dispensable for the growth of the cell. The 11th Thermophiles Conference, September 12-16, 2018, Kyoto, Japan.

7.Huang L. A novel primase subunit essential for efficient primer synthesis by the eukaryotic-type primase from Sulfolobus solfataricus. 13th Thermophiles Conference, August 31 - September 4, 2015, Santiago, Chile.

8.Huang L. Efficient primer synthesis by a heterotrimeric primase from Sulfolobus. Molecular Biology of Archaea 4, May 19 - 22, 2014, Paris, France.

9.Huang L. A novel primase subunit from Sulfolobus solfataricus. The 12th Thermophiles Conference, September 8-13, 2013, Regensburg, Germany.

10.Huang L, Chu Y, Zhang Z. A crenarchaeal protein lysine methyltransferase with relaxed substrate specificity. The 2013 Gordon Research Conference on Archaea: Ecology, Metabolism & Molecular Biology, July 28 - August 2, 2013, Lucca, Italy.

11.Huang L. Discovery and characterization of a novel protein lysine methyltransferase from Sulfolobus islandicus. The 9th International Congress on Extremophiles, September 10 - 13, 2012, Sevilla, Spain.

12.Huang L. New insights into the interaction between the crenarchaeal chromatin protein Cren7 and DNA. Gordon Research Conference, July 31 - August 5, 2011, Waterville Valley, USA.

13.Huang L. The Sac10b protein family of Archaea. The 7th International Congress on Extremophiles, September 7 - 11, 2008, Cape Town, South Africa.

14.Huang L. DNA synthesis by DNA polymerase B1 from Sulfolobus solfataricus: characteristics and modulation by other protein factors. The 9th Thermophiles Conference, September 24 - 27, 2007, Bergen, Norway.

15.Huang L, Wu K, Lai X, Guo X, Hu J, Xiang X. Physical and Functional Interaction between Primase and Replication Factor C in the Hyperthermophilic Archaeon Sulfolobus solfataricus. The 6th International Congress on Extremophiles, September 17 - 21, 2006, Brest, France.

16.Huang L. Interplay between primase and replication factor C in the hyperthermophilic archaeon Sulfolobus solfataricus. International Workshop on Extremophiles. August 20 - 23, 2006, Beijing, China.

17.Huang L. Adaptation of life to high temperature: lessons from Sulfolobus. The 8th Thermophiles Conference, September 18 - 22, 2005, Crowne Plaza Surfers Paradise, Gold Coast, Queensland, Australia.

主要获奖情况:

2022 美国微生物科学院（AAM）Fellow

2016 全国优秀科技工作者

2016 中国科学院朱李月华优秀教师奖

2010 中国侨联科技创新人才奖

2006 基金委创新群体学术带头人

2003 中国科学院“百人计划”入选者

2002 全国科普先进工作者

1999 基金委杰出青年基金获得者